Paper Title

CONDENSATION, CHARGE TRANSFER AND ION ASSOCIATION COMPLEX FORMATION REACTIONS FOR THE SPECTROSCOPIC DETERMINATION OF SPIRAMYCIN IN PURE AND PHARMACEUTICAL FORMULATION

Abstract

Eight simple, accurate and more sensitive spectrophotometric methods have been developed for the determination of Spiramycin (SPI), in both pure and pharmaceutical preparations. The method M1 p-dimethylaminobenzaldehyde (PDAB) and M2 Vanillin are condensation reactions with Spiramycin. The method M3 Chloranil and method M8 2,6-dichloroquinone N-chlorimide (DCQC) forms charge transfer complex with SPI. The method M4 wool fast blue (WFB) and M5 Bromo Cresol Green (BCG) involves ion association complex formation with Spiramycin. The method M6 Folin-Ciocalteu reagent (F-C Reagent) the color formation with Spiramycin is due to oxidation - reduction and method M7 Citric acid/Ac2O forms colour complex with Spiramycin. Regression analysis of Beer’s law plots showed good correlation in the concentration range of 5.0 - 50, 5.0 - 50, 2.5 - 15, 2.5 - 15, 5.0 - 30, 2.5 - 10, 2.5 - 15 and 2.5 - 15 and the corresponding molar absorptivity values are 1.4247 x 104, 1.256 x 104, 4.324 x 104, 5.4967 x 104, 6.1543 x 103, 7.0226 x 104, 5.1594 x 104 and 3.389 x104 for methods M1, M2, M3, M4, M5, M6, M7 and M8 respectively. All variables have been optimized and the results were statistically compared with those of literature methods by employing the student’s T-test and F-test. No interference was observed from excipients normally added to the tablets.
KEYWORDS : Spiramycin (SPI), Visible spectrophotometry; Vanillin, Chloranil, p- Dimethyl amino benzaldehyde